The general objectives of SALIVAGES are:

  1. To develop innovative technological approaches for the detection of the main salivary AGEs modulated by diets, in comparison to AGEs patterns detectable in urine and serum.
  2. To test the role of salivary AGEs as early and easily accessible biomarkers of changes in health status and/or the risk of developing diet-related diseases.

The specific objectives of SALIVAGES will be:

  • To develop in vivo models suitable for studying diet-induced AGEs production.
  • To mechanistically identify the pathogenic link between AGEs overproduction and related excessive inflammatory response.
  • To validate AGEs and inflammatory-related products as biomarkers in experimental models of metabolic diseases by metabolomics approaches integrated with advanced analytical methods for analytes separation/identification and biochemical assays.
  • To propose innovative technologies of biosensors for the salivary detection of AGEs.
  • To explore salivary volatilome to reveal informative features correlated with AGEs distribution.
  • To investigate glycosylation changes in biological samples in response to AGEs and thereby potentially identify putative glycobiomarkers using innovative methods for glycomic analyses.
  • To verify whether data emerged from experimental work are relevant for humans, by comparative evaluation of AGEs and related biomarkers in human saliva, serum and urine using biosensors, metabolomics tools and biochemical assays.